• Studies in developmental biology have identified key regulat

  2018-10-20

  

  Studies in developmental biology have identified key regulators of morphogenetic properties, and have indicated where endogenous EMF is located in the action potentials of nerves and heart tissue, and in skeletal muscle vibrations, with frequencies elicited by rhythmic activities throughout the huma

• We and oth http www GENS BIO COM images s

  2018-10-20

  

  We and others have shown that hiPSC have the ability to differentiate into different cell types belonging to the motor system, especially to lower motoneurons (Stockmann et al., 2011). In this context, a co-culture system using hiPSC derived motoneurons and differentiated primary myotubes represents

• br Materials and methods br Author disclosure

  2018-10-20

  

  Materials and methods Author disclosure statement Acknowledgments We are grateful to Prof. Angel Raya for his help and advice with iPS cell generation. This work was supported by grants from the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER) (grant 13-717/132.05

• br Mat http www RO

  2018-10-20

  

  Material andmethods Results anddiscussion Conclusions Here we report a comprehensive and unique transcriptomic study of the kinetics of gene motor protein during ASC adipogenesis in vitro. Despite the limitation of the study design including the use of relatively late passage ASCs and the

• The sorted BMSC and HSPC populations were observed

  2018-10-20

  

  The sorted BMSC and HSPC populations were observed to exist in distinct states of the cell cycle, equally important for their functions as stem cells. The ARCA gene expression studies further ascertained the cellular physiology of both the sorted cell populations. Our results also coincide with stud

• The four cell lines present the typical morphology

  2018-10-20

  

  The four cell lines present the typical morphology of pluripotent stem cells, growing in compact colonies, with tightly packed thrombin inhibitors (Fig. 1B). They express the pluripotency markers SSEA4, SSEA3, TRA-1-81, TRA-1-60 and OCT3/4, assessed by flow cytometry (Fig. 2A), and OCT4, REX1, NANO

• Although the present study focuses predominantly on keratino

  2018-10-20

  

  Although the present study focuses predominantly on keratinocytes, it has been known for many years that fibroblasts play an important role in wound healing and in remodeling the extracellular matrix. Thus, an ideal bioengineered graft will always need to incorporate fibroblasts as well as keratinoc

• In our long term cultures of human iPSC CMs the

  2018-10-20

  

  In our long-term cultures of human iPSC CMs, the cTnI:ssTnI ratio stalled at ∼2:98, making it fetal-like with regard to the TnI isoform profile. This stalled marker of development was observed using different differentiation protocols, in multiple hiPSC lines (five hiPSC-CMs line obtained from diffe

• br Experimental Procedures br Author Contributions

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction The mammary gland is a unique organ because its functional development and differentiation are completed postnatally. Ductal branching and elongation take place during puberty, whereas lobulo-alveolar development a

• It is thought that a better understanding of the

  2018-10-20

  

  It is thought that a better understanding of the molecular and cellular mechanisms that regulate the emergence and maintenance of long-term repopulating hematopoietic stem RG7112 (HSCs) during embryonic development would aid in the development of optimal protocols to generate such cells in vitro fro

• br Materials and methods br Acknowledgments This work was

  2018-10-20

  

  Materials and methods Acknowledgments This work was supported by Spanish Ministry of Economy and Competitiveness and European Regional Development Fund (grant numbers SAF2013-43005-R and SAF2016-76004-R). The authors thank INDEPF (Instituto de investigación y desarrollo social de enfermedades

• Monosynaptic tracing using modified rabies virus has previou

  2018-10-20

  

  Monosynaptic tracing using modified rabies virus has previously been successfully used to trace existing neuronal circuits in vivo (Wall et al., 2013; Watabe-Uchida et al., 2012) and also to map the integration of newborn neurons in the olfactory bulb and hippocampus (Deshpande et al., 2013; Vivar e

• In accordance with recently published data

  2018-10-20

  

  In accordance with recently published data, our approach involves use of target-specific neurogenic factors for direct conversion toward a neural fate. Findings of other groups have provided strong evidence of reprogramming of mouse and human fibroblasts to functional neurons following forced hemato

• In proof of principle experiments we examined

  2018-10-20

  

  In proof-of-principle experiments, we examined the response of hESC-derived CMs to verapamil and isoproterenol, two drugs commonly used in cardiovascular research. Single CMs exhibited a significantly reduced calcium transient amplitude associated with a negative inotropic response to treatment with

• SOX is an essential factor in the maintenance

  2018-10-20

  

  SOX2 is an essential factor in the maintenance of both human and murine pluripotency; however, it is upregulated, along with other pluripotency factors, in murine PGCs but not hPGCs. In this study we have clarified the mechanism of its repression in the hPGC-derived TCam-2 SEM atpase inhibitor and

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