Although the present study focuses predominantly on keratino

2018-10-20

Although the present study focuses predominantly on keratinocytes, it has been known for many years that fibroblasts play an important role in wound healing and in remodeling the extracellular matrix. Thus, an ideal bioengineered graft will always need to incorporate fibroblasts as well as keratinoc

In our long term cultures of human iPSC CMs the

2018-10-20

In our long-term cultures of human iPSC CMs, the cTnI:ssTnI ratio stalled at ∼2:98, making it fetal-like with regard to the TnI isoform profile. This stalled marker of development was observed using different differentiation protocols, in multiple hiPSC lines (five hiPSC-CMs line obtained from diffe

br Experimental Procedures br Author Contributions

2018-10-20

Experimental Procedures Author Contributions Acknowledgments Introduction The mammary gland is a unique organ because its functional development and differentiation are completed postnatally. Ductal branching and elongation take place during puberty, whereas lobulo-alveolar development a

It is thought that a better understanding of the

2018-10-20

It is thought that a better understanding of the molecular and cellular mechanisms that regulate the emergence and maintenance of long-term repopulating hematopoietic stem RG7112 (HSCs) during embryonic development would aid in the development of optimal protocols to generate such cells in vitro fro

br Materials and methods br Acknowledgments This work was

2018-10-20

Materials and methods Acknowledgments This work was supported by Spanish Ministry of Economy and Competitiveness and European Regional Development Fund (grant numbers SAF2013-43005-R and SAF2016-76004-R). The authors thank INDEPF (Instituto de investigación y desarrollo social de enfermedades

Monosynaptic tracing using modified rabies virus has previou

2018-10-20

Monosynaptic tracing using modified rabies virus has previously been successfully used to trace existing neuronal circuits in vivo (Wall et al., 2013; Watabe-Uchida et al., 2012) and also to map the integration of newborn neurons in the olfactory bulb and hippocampus (Deshpande et al., 2013; Vivar e

In accordance with recently published data

2018-10-20

In accordance with recently published data, our approach involves use of target-specific neurogenic factors for direct conversion toward a neural fate. Findings of other groups have provided strong evidence of reprogramming of mouse and human fibroblasts to functional neurons following forced hemato

In proof of principle experiments we examined

2018-10-20

In proof-of-principle experiments, we examined the response of hESC-derived CMs to verapamil and isoproterenol, two drugs commonly used in cardiovascular research. Single CMs exhibited a significantly reduced calcium transient amplitude associated with a negative inotropic response to treatment with

SOX is an essential factor in the maintenance

2018-10-20

SOX2 is an essential factor in the maintenance of both human and murine pluripotency; however, it is upregulated, along with other pluripotency factors, in murine PGCs but not hPGCs. In this study we have clarified the mechanism of its repression in the hPGC-derived TCam-2 SEM atpase inhibitor and

Hello world!

2018-07-29