• Research into embryogenesis and nervous system development

  2018-11-08

  

  Research into embryogenesis and nervous system development has been instrumental to the identification of factors required for cell specification. Using information gleaned from these studies, many groups have developed induction protocols to instruct hESCs and iPSCs to become a variety of neural ce

• In the present study our data generated

  2018-11-08

  

  In the present study, our data generated from the biochemical assays (Fig. 7) and functional studies (Figs. 2–5) indicated that the C-terminal PDZ motif of CXCR2 is important for EPC motility and angiogenic activity both in vitro and in vivo. Our findings suggested that a PDZ-based CXCR2 macromolecu

• Immunohistochemical studies revealed increase in the proport

  2018-11-08

  

  Immunohistochemical studies revealed increase in the proportion of CD44highCD24low cell type from low in normal tissues to quite high in tumours. This is interesting as we know that Cancer Stem Cells are the actual tumour initiating subpopulation and can expand depending on its evolutionary landscap

• br Materials and methods br

  2018-11-08

  

  Materials and methods Acknowledgements Mef2c-AHF-Cre mice were generously provided by Dr. Brian L. Black. We thank Mamen Martín and Sandra Rodríguez (Molecular Cytogenetics Unit, CNIO) for technical advice; and the Imaging Core Facility for the confocal analysis (CIMA, University of Navarra).

• Expression of pluripotency markers was examined by immunocyt

  2018-11-08

  

  Expression of pluripotency markers was examined by immunocytochemistry staining, using hydroxychloroquine sulfate against human OCT3/4, E-CADHERIN, and NANOG (Fig. 1A). The in vitro spontaneous differentiation potential towards the three germ layer of the BIOT-0726-LOAD iPSC line was demonstrated by

• Indeed both hAT MSC and hiPS

  2018-11-08

  

  Indeed, both hAT-MSC and hiPS-MSC baACL implants, when validated in a swine ACL excision model, resulted in proper ACL tissue formation. In the osseous zones, both in the tibia and the femur, the fibres of the biomaterial were surrounded by newly formed tissue that was encapsulated within mature bon

• br Acknowledgments We would like to thank

  2018-11-08

  

  Acknowledgments We would like to thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the plasmids for reprogramming and Dr. Feng Zhang for providing the plasmid for gene editing. We thank Ida Jørring and Bente Smith Thorup for excellent technical assistance in the cell culture. We tha

• Other cell extrinsic mechanisms may lead

  2018-11-08

  

  Other cell-extrinsic mechanisms may lead to a progressive loss of WT ep4 through an active process that eliminates the least fit populations. Such processes occur during normal development in the mammalian embryo, in which relatively unfit populations are deleted through apoptosis (Clavería et al.,

• In their letter Sadek et

  2018-11-08

  

  In their letter, Sadek et al. refer to other studies showing robust and reproducible regeneration. However, none of the studies published to date (Haubner et al., 2012; Heallen et al., 2013; Jesty et al., 2012; Naqvi et al., 2014; Strungs et al., 2013) actually performed apex resection on postnatal

• Expression of Sox alone was sufficient to induce neurogenesi

  2018-11-08

  

  Expression of Sox2 alone was sufficient to induce neurogenesis without requirement for coexpression of a proneural transcription factor. This is surprising, as Sox2 function in neural stem cells is to promote self-renewal rather than neuronal differentiation (Graham et al., 2003; Pevny and Nicolis,

• br Results br Discussion To identify

  2018-11-08

  

  Results Discussion To identify global trends in the application of hESCs and hiPSCs in research, we established a curated database of published primary research conducted with these cox 2 inhibitors between 2008 and 2013, and performed a thorough analysis of studies involving only hESCs or onl

• br Resource table Resource details Macaca

  2018-11-08

  

  Resource table. Resource details Macaca fascicularis iPS TCEP ATCi-MF1 were generated from adult skin-derived fibroblasts by transduction with non-integrative Sendai viruses coding for the reprogramming factors OCT3/4, KLF4, SOX2 and c-MYC (Ban et al. 2011; Zapata-Linares et al. 2016). Transduc

• As an initial application of our RNA

  2018-11-08

  

  As an initial application of our RNA-seq datasets for discovery of regulatory mechanisms in huSCs, we compared transcriptomes of quiescent huSCs to those of activated huSCs to identify loci that were differentially expressed. In quiescent huSCs, we identified 998 highly expressed genes, including 1

• Researchers are usually aware of the tissue

  2018-11-08

  

  Researchers are usually aware of the tissue that was initially used for isolation of MSCs, but there is evidence that accidental interchange of samples or contaminations with other purchase GSK126 can occur (Garcia et al., 2010; Torsvik et al., 2010). For established cell lines, some contaminations

• Recently we found that vesicular

  2018-11-08

  

  Recently, we found that vesicular monoamine transporter 2 (VMAT2) regulates monoamine (including dopamine) storage by acting as a negative regulator, such that high monoamine levels inhibit the progression from Pdx1+ pancreatic progenitor d-biotin to Neurog3+ endocrine progenitor cells of ESC-deriv

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